NOHA HASSAN KHALIL IBRAHIM

06/12/2023

https://www.mdpi.com/1999-4923/15/12/2734

NOHA HASSAN KHALIL IBRAHIM

Mariam Gonaid

13/10/2023

https://fjps.springeropen.com/articles/10.1186/s43094-023-00538-5

NOHA HASSAN KHALIL IBRAHIM

Alshaimaa M. Almehmady ; Hanaa Alkharobi ; Sameh S. Elhady

29/09/2023

https://www.mdpi.com/2218-1989/13/10/1044

NOHA HASSAN KHALIL IBRAHIM

Mohamed S. Elnaggar; Abdel Nasser B. Singab

17/05/2023

https://link.springer.com/article/10.1007/s00203-023-03580-2

NOHA HASSAN KHALIL IBRAHIM

Haidy A. Gad, Manar O. Lashkar, Roaa M. Khinkar, Mohmmad Y. Wani

06/11/2022

https://www.mdpi.com/2073-4395/12/11/2763

NOHA HASSAN KHALIL IBRAHIM

Sameh S. Elhady; Reem M. Diri; Mostafa I. Fekry; Mokhtar Bishr; Osama Salama; Soheir M. El-Zalabani

23/09/2022

https://mdpi-res.com/d_attachment/agronomy/agronomy-12-02278/article_deploy/agronomy-12-02278.pdf?version=1663920367

NOHA HASSAN KHALIL IBRAHIM

Haidy A. Gad; Rano Z. Mamadalieva ; Gokhan Zengin; Basma Najar; Olim K. Khojimatov; Nawal M. Al Musayeib; Mohamed L. Ashour; Nilufar Z. Mamadalieva

23/08/2022

https://www.mdpi.com/1420-3049/27/17/5365

NOHA HASSAN KHALIL IBRAHIM

Haidy Gad; Nawal Al Musayeib; Mokhtar Bishr; Mohamed Ashour

20/04/2022

https://www.mdpi.com/2223-7747/11/9/1116

NOHA HASSAN KHALIL IBRAHIM

Mokhtar Bishr , Mohamed El-Degwy , Mohamed Abdelhady , Mohamed Amin and Osama Salama

01/02/2021

Background: Khella (Ammi visnaga Lam.) fruits (Apiaceae) are rich in furanochromones, mainly khellin and visnagin, and are thus incorporated in several pharmaceutical products used mainly for treatment of renal stones. Methods: The objective of this study was to compare the yield of khellin and visnagin obtained using different conventional solvents and supercritical fluid extraction (SCFE) with carbon dioxide (containing 5% methanol as co-solvent). Water, acetone and ethanol (30% and 95%) were selected as conventional solvents. Results: Highest extract yield was obtained from 30% ethanol (15.44%), while SCFE gave the lowest yield (4.50%). However, the percentage of furanochromones were highest in SCFE (30.1%), and lowest in boiling water extract (5.95%). HPLC analysis of conventional solvent extracts showed other coumarins that did not appear in supercritical fluid extraction chromatogram due to non-selectivity of solvent extraction. Ammi visnaga extracts as well as standard khellin and visnagin were tested for their cytotoxic activity using sulforhodamine B assay on breast cancer (MCF-7) and hepatocellular carcinoma (Hep G2) cell lines. Results revealed a strong cytotoxic activity (IC50 < 20 µg/mL) for the SCFE and standard compounds (khellin and visnagin) (IC50 ranging between 12.54 ± 0.57 and 17.53 ± 1.03 µg/mL). However, ethanol and acetone extracts had moderate cytotoxic activity (IC50 20–90 µg/mL) and aqueous extract had a weak activity (IC50 > 90 µg/mL). Conclusions: Thus, supercritical fluid extraction is an efficient, relatively safe, and cheap technique that yielded a more selective purified extract with better cytotoxic activity.

NOHA HASSAN KHALIL IBRAHIM

MAHA EL-DEMELLAWY, MARWA MOHAMED , MOHAMED MAHROUS, ABEER ABDELWAHAB, SAHAR FIKRY, DOAA GHAREEB, OSAMA SALAMA AND MAE SELEEM

01/01/2021

HCV represents a global health problem. In Egypt, 20% of the population are infected with HCV. During the infectious phase, HCV attenuates the immunity by producing specific HCV immune clearance. Blue green algae (BGA), especially Spirulina species, was studied pertaining to its effect on the human immune response in HCV. The lysate of non-toxic BGA was isolated and identified as Nostoc sp EGY (NE). IL-4, IL-10, IL-12, INF-γ and TNFα mRNAs and their expressed protein were assessed using RT-PCR and ELISA, respectively. The prepared lysate induced immune stimulation of peripheral blood mononuclear cells (PBMC) at a concentration range of 10-500µg/ml and viral clearance at 1 ng/ml. Moreover, concentrations of this crude lysate ranging from 100µg10mg/ml stimulated normal human neutrophils to ingest and kill live Candida in an in-vitro test. IL-4, 10 and 12 proteins and their mRNA expression were significantly lower in HCV infected/NE lysate-treated PBMC, yet higher than HCV infected/NE lysate-untreated PBMC. This was in contrast to the levels of INF-γ and TNF-α which showed significant decrease in HCV infected/NE lysate-treated PBMC relative to HCV infected/NE lysateuntreated PBMC. These findings give further insight regarding the effect of NE lysate on improving the immune response, thus presenting a promising safe and effective immune candidate for HCV treatment.

NOHA HASSAN KHALIL IBRAHIM

Lamya El-Jalel , Miriam Yousif and Mariam Gonaid

01/06/2020

Background: Several agricultural or environmental factors affect plants’ chemical and pharmacological properties. Methods: In this study, the essential oil of Libyan Satureja thymbra was isolated from plants collected during two successive years at two different altitudes; Wasita (WEO) and Safsaf (SEO), 156 and 661 m above sea level, respectively. Results: GC/MS allowed the identification of 21 and 23 compounds, respectively. Thymol prevailed in WEO (26.69%), while carvacrol prevailed in SEO (14.30%). Antimicrobial activity was tested by agar-well diffusion method, and MIC/MLC values were determined by broth dilution method. Values of MIC/MLC were 0.125/0.25 μg/ml for SEO against S. aureus, P. mirabilis and K. pneumonia and for WEO against B. subtilus. It was observed that plants growing at lower altitude in Wasita locality had better antifungal activity, while those growing at higher altitude at Safsaf locality had better antibacterial activity. Both essential oils had a better anthelmintic activity than the standard piperazine citrate against a tested earthworm. However, SEO oil had a significantly higher anthelmintic activity than WEO. Cytotoxicity of the oils tested using SRB assay on human breast cancer (MCF-7) and colon cancer cell lines (HCT-116) showed better activity for SEO, especially against HCT-116 with IC50 2.45 ± 0.21 μg/ml. Conclusions: Thus, altitude is an important factor that should be considered as it affected the yield, composition and biology of the plant extracts."

NOHA HASSAN KHALIL IBRAHIM

Bishr M, Desouky S

01/01/2020

NOHA HASSAN KHALIL IBRAHIM

Fikry S

01/01/2020

NOHA HASSAN KHALIL IBRAHIM

Khalil NH, Fikry SM, Salama OM.

01/12/2019

NOHA HASSAN KHALIL IBRAHIM

Sahar Fikry

01/03/2019

Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganisms. GC/MS of OV EO allowed the identification of 32 compounds representing 99.94% of the oil. The two major identified compounds were terpinen-4-ol (38.35%) and trans-sabinene hydrate (10.06%). Different methods were employed to illustrate the biostatic activity of OV EO. Results of the biostatic studies on OV EO using agar and broth dilution methods showed that Staphylococcus aureus (S. aureus) was the most sensitive organism; with a Minimum inhibitor concentration (MIC) 1.18 mg/ml. Agar diffusion method showed that the highest activity was observed against Bordetella bronchiseptica (Br. bronchiseptica), Saccharomyces cerevisiae (S. cerevisiae), Bacillus subtilis (B. subtilus) and Staphylococcus epidermidis (S. epidermidis) with inhibition zones 38 ± 1.5, 29.5 ± 0.8, 26.9 ± 0.9 and 26.9 ± 1.1 mm, respectively. Studying the dynamics of 1% v/v OV essential oil emulsion over a period of 6 h revealed that Escherichia coli (E. coli), B. subtilis, S. epidermidis and S. cerevisiae had the fastest response. Also increasing concentrations of OV oil emulsion increased the rate of cell killing and the duration of growth lag phase increased correspondingly. These data indicated that OV EO produces a concentration and time-dependent antimicrobial activity.

NOHA HASSAN KHALIL IBRAHIM

Mohamed Ashour, Abdel Naser Singab

01/06/2018

The hexane extract and the hydro-distilled essential oil from red carrot fruits (Daucus carota var. boissieri) were evaluated for their cytotoxic activity against human tumor breast cell lines (MCF-7). Cell viability was evaluated by MTT assay. The extract exhibited good cytotoxic activity shown through its low IC50 (9.12 ± 0.58 μg/ml) against the standard 5-Flououracil (8.46 ± 0.63 μg/ml). Phytochemical investigation of the hexane extract using column chromatography yielded three compounds; 8-methoxypsoralen (1), α-asarone (2) and 3,4,5-trimethoxy-benzaldehyde (3), a compound isolated for the first time from D. carota and from family Apiaceae. Structure elucidation of the isolated compounds was carried out on the basis of their spectral data analysis (IR, MS, 1H NMR an 13C NMR) The three isolated compounds were evaluated for their cytotoxic activity using the same conditions. Only compound (1) exhibited good cytotoxic activity (IC50; 9.38 ± 0.78 μg/ml), compound (2) had moderate activity (46.12 ± 1.31 μg/ml), while compound (3) had no cytotoxic activity (100.6 ± 3.11 μg/ml). These compounds need to be more investigated against other cell lines; also they are considered as a good substrate for future SAR study and modifications to produce more potent cytotoxic derivatives

NOHA HASSAN KHALIL IBRAHIM

Mohamed Ashour, Sahar Fikry, Abdel Naser Singab

01/06/2018

Antimicrobial properties of plants essential oils are continuously investigated to use them as potential drug candidates to overcome the problem of microbial drug resistance. The aim of this research is to study the antimicrobial effects of the essential oils of ten Apiaceous fruits [Pimpinella anisum L. (anise), Carum carvi L. (caraway), Apium graveolens L. (celery), Coriandrum sativum L. (coriander), Cuminum cyminum L. (cumin), Anethum graveolens L. (dill), Foeniculum vulgare L. (fennel), Petroselinum crispum L. (pasley), Daucus carota L. var. sativus (yellow carrot) and Daucus carota L. var. boissieri (red carrot)]. Results of agar-well diffusion method revealed that the maximum inhibition zones were obtained with cumin, coriander and caraway oils against the standard bacterial strains Escherichia coli, Bordetella bronchiseptica followed by Staphylococcus aureus. Results of viable count time-kill method revealed that coriander oil had the highest antimicrobial activity with more than 99.99% killing of the exposed cells of the standard E. coli and Bordetella bronchiseptica standard strains. GC/MS was carried out to identify the chemical composition of the most active oils. The percentage of identified compounds by GC/MS was 92.5%, 99.43% and 98.66% for cumin, coriander and caraway oils, respectively. Monoterpenes were the most abundant components in the three oils.

NOHA HASSAN KHALIL IBRAHIM

Mostafa Fekry, Mokhtar Bishr, Soheir El-Zalabani

01/02/2018

Polyphenolic compounds are considered valuable secondary plant metabolites owing to the myriad of biological activities they exert. This study aimed to investigate the effect of applying various concentrations of the plant growth regulator, salicylic acid (SA), on Thymus vulgaris L. while subjecting the plant to decreasing amounts of irrigation water. The following parameters were monitored; total polyphenolic and flavonoid content, yield and composition of the essential oil, and antioxidant activity of the alcoholic extracts. Drought alone significantly (P < 0.05) increased the polyphenolic and flavonoid content, yield of the essential oil and antioxidant activity. The total flavonoid content in control plants was 6.1 ± 0.3 mg/gm dry weight calculated in terms of rutin equivalent. However, in drought stressed plants, (irrigated at 25% of the field capacity) sprayed with 3 mM SA, the flavonoid content increased to 32.1 ± 0.1 mg/gm dry weight calculated in terms of rutin equivalent. Moreover, the total phenolic content increased from 8.5 ± 0.3 to 68.5 ± 1.2 mg/gm dry weight calculated in terms of gallic acid in the same test plants. Radical scavenging activity, using DPPH assay, was measured for the different plant treatments. A decrease from 74.4 ± 0.4 μg/ml to 36.6 ± 0.9 μg/ml of IC50 was recorded in the drought stressed plants (25% FC) sprayed with 3 mM SA compared with the control plants. The variability in polyphenolic composition between the control plants and plants with the highest total polyphenolic content was investigated by UPLC-ESI-MS/MS. Rosmarinic acid was detected as the major component in samples from both treatments, with a higher percentage observed upon subjecting the plant to the test conditions (25% FC and sprayed with 3 mM SA). The highest yield of the essential oil (1 ± 0.06 %v/w) was obtained from drought stressed plants (25% FC) sprayed with 2 mM SA. GC/MS analysis of oil samples revealed that the Thymol content increased with drought stress, while that of p-cymene decreased. However, an increase of p-cymene was witnessed as a result of SA spraying.

NOHA HASSAN KHALIL IBRAHIM

S Fikry, A NaserSingab

01/05/2016

Antimicrobial properties of essential oils of plants are continuously being investigated in order to use them as potential drug candidates to overcome the problem of microbial drug resistance [1]. The aim of this research is to study the antibacterial and antifungal effects of ten traditional Apiaceous essential oils; Pimpinella anisum L. (Anise), Carum carvi L. (Caraway), Apium graveolens L. (Celery), Coriandrum sativum L. (Coriander), Cuminum cyminum L. (Cumin), Anethum graveolens L. (Dill), Foeniculum vulgare (Fennel), Petroselinum crispum L. (Parsely), Daucus carota L. var. sativus (Red Carrot) and D. carota L. var. boissieri (Yellow Carrot).GC/MS was carried out to identify the chemical composition of the most active antimicrobial essential oils. Results of agar-well diffusion method revealed that the maximum inhibition zones were obtained with cumin, coriander and caraway oils against the standard bacterial strains Escherichia coli, Bordetella bronchiseptica followed by Staphylococcus aureus. On the other hand, the remaining essential oils showed much smaller inhibition zones or no inhibition at all. The three most active essential oils; cumin, caraway and coriander were selected to confirm their antibacterial activity by using viable count time-kill method. Results of this experiment revealed that the coriander essential oil had the highest antimicrobial activity with more than 99.999% killing of the exposed cells of the standard E. coli and Bordetella bronchiseptica standard strains during 5 minutes exposure. These results are in accordance with previously published data [2]. The percentage of identified compounds by GC/MS was 92.5, 99.43 and 98.66 for the cumin, coriander and caraway oils respectively. Monoterpenes were the most abundant components in the three oils.

NOHA HASSAN KHALIL IBRAHIM

Ashour M, Singab AN, Salama O

01/03/2015