Amr Maged Ibrahim Abdelbaky

20/07/2023

https://www.mdpi.com/1999-4923/15/7/1990

Amr Maged Ibrahim Abdelbaky

06/12/2022

https://www.tandfonline.com/doi/full/10.1080/10717544.2022.2152135

Amr Maged Ibrahim Abdelbaky

25/04/2022

https://www.tandfonline.com/doi/full/10.1080/10717544.2022.2069878

Amr Maged Ibrahim Abdelbaky

25/03/2022

https://www.sciencedirect.com/science/article/abs/pii/S037851732200103X

Amr Maged Ibrahim Abdelbaky

Suzan M Mansour, Rehab N Shamma, Kawkab A Ahmed, Nirmeen A Sabry, Gamal Esmat, Azza A Mahmoud, Amr Maged

01/07/2021

Introduction: SARS-CoV-2 replication in cell cultures has been shown to be inhibited by ivermectin. However, ivermectin's low aqueous solubility and bioavailability hinders its application in COVID-19 treatment. Also, it has been suggested that best outcomes for this medication can be achieved via direct administration to the lung.Objectives: This study aimed at evaluating the safety of a novel ivermectin inhalable formulation in rats as a pre-clinical step.Methods: Hydroxy propyl-β-cyclodextrin (HP-β-CD) was used to formulate readily soluble ivermectin lyophilized powder. Adult male rats were used to test lung toxicity for ivermectin-HP-β-CD formulations in doses of 0.05, 0.1, 0.2, 0.4 and 0.8 mg/kg for 3 successive days.Results: The X-ray diffraction for lyophilized ivermectin-HP-β-CD revealed its amorphous structure that increased drug aqueous solubility 127-fold and was rapidly dissolved within 5 s in saline. Pulmonary administration of ivermectin-HP-β-CD in doses of 0.2, 0.4 and 0.8 mg/kg showed dose-dependent increase in levels of TNF-α, IL-6, IL-13 and ICAM-1 as well as gene expression of MCP-1, protein expression of PIII-NP and serum levels of SP-D paralleled by reduction in IL-10. Moreover, lungs treated with ivermectin (0.2 mg/kg) revealed mild histopathological alterations, while severe pulmonary damage was seen in rats treated with ivermectin at doses of 0.4 and 0.8 mg/kg. However, ivermectin-HP-β-CD formulation administered in doses of 0.05 and 0.1 mg/kg revealed safety profiles.Conclusion: The safety of inhaled ivermectin-HP-β-CD formulation is dose-dependent. Nevertheless, use of low doses (0.05 and 0.1 mg/kg) could be considered as a possible therapeutic regimen in COVID-19 cases.

Amr Maged Ibrahim Abdelbaky

slam M Adel, Mohamed F ElMeligy, Mohamed EA Abdelrahim, Amr Maged, AbdelFattah A Abdelkhalek, Azza MM Abdelmoteleb, Nermeen A Elkasabgy

01/04/2021

Purpose The goal was to directly deliver curcumin, a natural polyphenolic anticancer and anti-inflammatory compound, to the lung tissues with minimal systemic exposure through the fabrication of proliposomes, overcoming its poor aqueous solubility and oral bioavailability. Methods Nano-spray drying was employed to prepare proliposomes using hydroxypropyl beta-cyclodextrin as a carrier. Lecithin and cholesterol were used as lipids, stearylamine and Poloxamer 188 were added as positive charge inducer and a surfactant, respectively. Different characterization parameters were evaluated like percentage yield, entrapment efficiency, drug loading, aerodynamic particle size, in vitro release besides morphological examination. Cytotoxicity studies on cell line A549 lung tumor cells as well as in vivo lung pharmacokinetic studies were also carried. Results The optimized formulations showed superior aerosolization properties coupled their enhanced ability to reach deep lung tissues with a high % of fine particle fraction. Cytotoxicity studies using MTT assay demonstrated enhanced growth inhibitory effect on lung tumor cells A549 and significant reduction of proinflammatory cytokines such as tumor necrosis factor-α, interleukin-6 and interleukin-10 compared to the pure drug. Results of lung pharmacokinetic tests confirmed the superiority of proliposomal curcumin over curcumin powder in both, the rate and extent of lung tissue absorption, as well as the mean residence time within the lung tissues. Conclusion The pulmonary delivery of curcumin-loaded proliposomes as dry powder provides a direct approach to lung tissues targeting while avoiding the limitations of the oral route and offering a non-invasive alternative to the parenteral one. Keywords: curcumin, proliposomes, cyclodextrin, dry powder inhalers, human epithelial cell line, pulmonary delivery

Amr Maged Ibrahim Abdelbaky

Nermeen A Elkasabgy, Azza A Mahmoud, Amr Maged

01/08/2020

In the recent decade, three-dimensional (3D) printers started to grow strongly in the field of drug delivery and personalized medicine, as they can tailor dosage forms according to the needs of each individual. This review gives an overview on the basic principles of layer-by-layer building of pharmaceutical dosage forms using different types of 3D printers. Also, the effect of infill percentage and pattern, raster orientation, layer thickness, thermal processing parameters on the printed formulations is highlighted. Additionally, the complex designs constructed by the 3D printers in order to modify the product shape, density, mucoadhesion and drug release are recapitulated. This review summarizes numerous applications for 3D printing in building drug-loaded structures including tablets, scaffolds, implants, microneedles, capsules, films, hydrogels, mouthguards, tubes, stents, vaginal suppositories and rings as well as in pediatric field. Finally, we suggest further investigational researches to aid in the widespread of 3D printing in the industrial pharmaceutical field. The 3D printing technology is expected to revolutionize drug delivery systems through customization of pharmaceutical formulations.

Amr Maged Ibrahim Abdelbaky

Salwa Salah, Nehad M. Abd-elmonsif, and Mahmoud M. Ghorab

01/07/2020

Amr Maged Ibrahim Abdelbaky

Abdelfattah A.Abdelkhalek, Azza A. Mahmoud, Salwa Salah, Mohamed M. Ammar, Mahmoud M. Ghorab

01/01/2019

In this study we explored the role of rosuvastatin calcium in skin regeneration as statins play important role in the field of tissue engineering. Chitosan hydrochloride was crosslinked with different weight ratios of collagen, β-glycerolphosphate and carboxymethyl cellulose to produce scaffolds by lyophilization technique. Subsequently, the fabricated scaffolds were examined for their morphology, water absorption capacity, water retention, friability and in-vitro drug release as well as in-vivo studies. The results revealed porous 3-D structured scaffolds with maximum water absorption values-ranging between 396 and 2993%. Scaffolds containing carboxymethyl cellulose revealed highest water absorption-values. In-vitro drug release results showed gradual drug release for 60 h with mean dissolution time-values (MDT) between 13 and 21 h. Combination of chitosan, collagen, carboxymethyl cellulose in weight ratio of 40:30:30, respectively achieved gradual disintegration of the scaffold in a simulating medium to an open wound after 4 days. This selected scaffold loaded with rosuvastatin revealed increase proliferation of human dermal fibroblasts compared to placebo scaffold. After 30 days of implantation of selected medicated scaffold loaded with/without mesenchymal stem cells and placebo scaffolds to induced wounds in Albino rats, enhanced skin regeneration and absence of scar formation for drug loaded scaffolds were observed. The histopathological study showed the advantage of stem cells-loaded scaffolds through the normal redistribution of collagen in the epidermal layer. In conclusion, rosuvastatin calcium and stem cells loaded in the tested scaffolds proved their potential effect in enhancing skin healing and regeneration.

Amr Maged Ibrahim Abdelbaky

Azza A. Mahmoud, Mahmoud M. Ghorab

01/08/2017

Nano-spray dryer is advanced instrument to produce a stable and spherical nanoparticles with high yield. In this study, econazole nitrate nanoparticles were formulated by nano-spray dryer using 1:1, 1:2 and 1:3 weight ratios of drug to hydroxypropyl-beta-cyclodextrin and stabilizer. The prepared samples were sprayedthrough nozzle size of 7.0 μm using 95ºC and 45ºC as inlet temperature and outlet temperatures, respectively. The prepared nanoparticles were evaluated for process yield and percent drug loading. Furthermore, the drug nanoparticles were dispersed in isotonic buffer solution and examined for drug release and their stability at room temperature. The spray dried particles were in the nano-range (148 to 294 nm) and their yield values ranged between 79.1 and 84.9 %. Increasing weight ratio of drug to hydroxypropyl-beta-cyclodextrin to 1:2 and 1:3 showed increases in percent drug release compared to formulation containing 1:1 weight ratio of drug to hydroxypropyl-beta-cyclodextrin. On the other hand, the prepared econazole nitrate nanosuspension containing 1:1 weight ratio of drug to hydroxypropyl-beta-cyclodextrin revealed best stability study during storage period at room temperature compared to other formulations. As a result of in-vitro drug release and stability studies, the optimum weight ratio of 1:1, drug to hydroxylpropyl-beta-cyclodextrin was chosen as a best weight ratio duo to its good balance between drug release and stability of drug loaded nanoparticles

Amr Maged Ibrahim Abdelbaky

Azza A. Mahmoud, Mahmoud M. Ghorab

01/03/2016

The effect of using methyl-β-cyclodextrin and hydroxypropyl-β-cyclodextrin as carriers for econazole nitrate nanoparticles prepared by nano spray dryer was explored in this work. Stabilizers, namely, poly(ethylene oxide), polyvinylpyrrolidone k30, poloxamer 407, Tween 80, and Cremophor EL, were used. The nano spray dried formulations revealed almost spherical particles with an average particle size values ranging from 121 to 1565 nm and zeta potential values ranging from −0.8 to −2.5 mV. The yield values for the obtained formulations reached 80%. The presence of the drug in the amorphous state within the nanosuspension matrix system significantly improved drug release compared to that for pure drug. Combination of hydroxypropyl-β-cyclodextrin with Tween 80 achieved an important role for preserving the econazole nanosuspension from aggregation during storage for one year at room temperature as well as improving drug release from the nanosuspension. This selected formulation was suspended in chitosan HCl to increase drug release and bioavailability. The in vivo evaluation on albino rabbit’s eyes demonstrated distinctly superior bioavailability of the selected formulation suspended in chitosan compared to its counterpart formulation suspended in buffer and crude drug suspension due to its mucoadhesive properties and nanosize. The nano spray dryer could serve as a one step technique toward formulating stable and effective nanosuspensions.