ASMAA ABDELKERIM MANDOUR KHADR

Eslam B Elkaeed ; Mohamed Hagras,

13/11/2023

https://www.future-science.com/doi/10.4155/fmc-2023-0065

ASMAA ABDELKERIM MANDOUR KHADR

Sherif Gamal; Gehad G. Mohamed; Said A. Salih; Menna I. Ezzeldin

23/10/2023

https://fjps.springeropen.com/articles/10.1186/s43094-023-00548-3

ASMAA ABDELKERIM MANDOUR KHADR

Riham A. El-Shiekh; Rehab M. S. Ashour; Mona M. Okba;Omnia Kutkat; Yassmin Moatasim; Rana Elshimy

26/09/2023

https://www.tandfonline.com/doi/abs/10.1080/14786419.2023.2261069

ASMAA ABDELKERIM MANDOUR KHADR

Gehad G. Mohamed; Said A. Salih

17/08/2023

https://fjps.springeropen.com/articles/10.1186/s43094-023-00519-8

ASMAA ABDELKERIM MANDOUR KHADR

17/08/2023

https://link.springer.com/article/10.1186/s43094-023-00519-8

ASMAA ABDELKERIM MANDOUR KHADR

Faisal K. Algethami;Huda Salem AlSalem;Mohammed Gamal;Nada Nabil;Hala E. Zaazaa;Mohamed A. Ibrahim

23/06/2023

https://doi.org/10.3390/pr11071888

ASMAA ABDELKERIM MANDOUR KHADR

23/02/2023

https://link.springer.com/content/pdf/10.1186/s43094-023-00464-6.pdf.

ASMAA ABDELKERIM MANDOUR KHADR

Nada Nabil ; Hala E. Zaazaa ; Munjed M. Ibrahim ; Mohamed A. Ibrahim

06/01/2023

https://www.mdpi.com/2297-8739/10/1/37

ASMAA ABDELKERIM MANDOUR KHADR

Ibrahim F. Nassar , Mohammed T. Abdel Aal , Mahmoud A. E. Shahin , Wael A. El-Sayed, , Maghawry Hegazy , Amr Mohamed Yehia, Ahmed Ismail, Mohamed Hagras , Eslam B. Elkaeedh

10/07/2022

https://pubmed.ncbi.nlm.nih.gov/35815597/

ASMAA ABDELKERIM MANDOUR KHADR

Ibrahim F. Nassar, *a Mohammed T. Abdel Aal,b Wael A. El-Sayed,cd Mahmoud A. E Shahin,Elsayed G. E. Elsakka, Mahmoud Mohamed Mokhtar, Maghawry Hegazy,Mohamed Hagras

17/05/2022

https://pubs.rsc.org/en/content/articlelanding/2022/ra/d2ra01968j

ASMAA ABDELKERIM MANDOUR KHADR

Nour E. A. Abd El-Sattar, Eman H. K. Badawy,aWafaa H. AbdEl-Hady,Mohamed I. Abo-Alkasem and Nasser S. M. Ismail

01/01/2021

Cyclin dependent kinase 2 (CDK2) inhibition is a well-established strategy for treating cancer. Differentseries of novel thiazolone (1, 7–9) together with fused thiazolthione (2–6, and 10) derivatives were designed,then synthesized and evaluated for their biological inhibitory activity against CDK2. Additionally, the cytotoxicityof the new compounds was explored against breast and colon cancer cell lines. The novel thiazoloneand the fused thiazolthione derivatives exhibited potent CDK2/cyclin A2 inhibitory effect of an IC50 valuesranging 105.39–742.78 nM. Amongst them compounds 4 and 6 revealed highest IC50 of 105.39 and 139.27 nM,respectively. Most compounds showed significant inhibition on both breast cancer and colon cancer cell lineswith IC50 range 0.54–5.26 and 0.83–278 μM, respectively. Further investigations involved flow cytometryanalysis on MCF-7 cancer cell line for compounds 5 and 7 which resulted in arrest cell-cycle at two phasesPre G1/G2-M and re-enforced apoptosis via activation of caspase-7. Molecular modeling simulation of thedesigned compounds revealed that they were well fitted into CDK2 active site and their complexes were stabilizedthrough the essential hydrogen bonding. Three dimensional quantitative structure activity relationship(3D QSAR) pharmacophore, and absorption, distribution, metabolism, excretion, and toxicity (ADMET)studies were also carried out showing proper pharmacokinetic and drug-likeness which aided in the predictionof the structure requirements responsible for the observed antitumor activity.

ASMAA ABDELKERIM MANDOUR KHADR

Nada Nabil, Hala E. Zaazaa and Mohamed Abdelkawy

01/08/2020

The heterocyclic compounds are extremely important with wide array of synthetic, pharmaceutical, and industrial applications. Heterocyclic-containing compounds have been reported for their broad spectrum of biological activities including antibacterial, antifungal, antiviral, antiprotozoal, and anthelmintic activity. Main text: Several techniques have been used for the quantitation of heterocyclic compounds in pharmaceutical samples such as high-performance liquid chromatography (HPLC) either equipped with UV-visible or fluorescence, in addition to liquid chromatography-mass spectroscopy, UV-visible spectrophotometry, and electrochemical techniques. This article reviewed several published methods that have been applied to detect and quantify some pharmaceutical drugs containing heterocyclic compounds focusing on four drugs: brinzolamide, timolol maleate, flumethasone pivalate, and clioquinol. Conclusion: From literature reviews, HPLC is the most widely used analytical technique for the quantitative analysis of the four selected drugs.

ASMAA ABDELKERIM MANDOUR KHADR

Elkady EF, Algethami FK, Aboelwafa AA, Farouk F.

01/02/2020

A recent fixed combination of amlodipine (AML) besylate, olmesartan (OLM) medoxomil and hydrochlorothiazide (HCT) has been marketed for the treatment of hypertension. The bioanalysis of this mixture is a challenge due to the diverse physicochemical properties. The aim of this study is to develop a method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous bioanalysis of the mixture in human plasma. A mobile phase of 10 mM ammonium formate containing 0.1% formic acid: methanol: acetonitrile (35:50:15,v/v/v) was recruited for the chromatographic separation of the mixture on a Zorbax SB-Aq (150×4.6 mm, 5 μm)column. The deutrated analogues of AML, OLM and HCT were used as the internal standards (IS). Polarity switching SRM transitions in positive-mode for AML, OLM, and negative-mode for HCT were applied for detection. Sequential double liquid-liquid extraction (SdLLE) was adopted for sample preparation. The developed method was fully validated and applied for the determination of AML, OLM and HCT following a single oral administration of 5/20/12.5 mg of AML, OLM and HCT, respectively tablets to healthy volunteers (n = 30). The developed method was linear (r2> 0.99), accurate (105 - 90%), precise (CV% <11.92) and specific for the determination of AML, OLM and HCT over the concentration range of 0.1–15, 5–1200 and 2–150 ng/mL. The adopted SdLLE resulted into reproducible extraction recoveries of 75%, 63% and 83% for AML, OLM and HCT, respectively. The pharmacokinetic analysis revealed uniform profile in the subjects which were comparable to previously reported results in other populations.

ASMAA ABDELKERIM MANDOUR KHADR

Asmaa A El-Zaher, Ehab Elkady,

01/05/2018

ASMAA ABDELKERIM MANDOUR KHADR

Ramzia El-Bagary, Asmaa Ahmed El-Zaher, Ehab Elkady

01/03/2016

Ciprofloxacin HCl (CIP) and Metronidazole (MET) are antibacterial drugs used in combination for treatment of mixed aerobic/anaerobic infections. An UPLC-MS/MS method was developed for the simultaneous estimation of CIP and MET in spiked human plasma using sildenafil citrate as an internal standard (IS). Protein precipitation was used for analyte extraction. The chromatographic separation was completed within 6 min using a mobile phase of 0.1% formic acid in water and acetonitrile (70: 30, v/v), Zorbax C18, 100 x 4.6 mm, 3.5 μm analytical column, at a flow rate of 0.5 mL min-1. Multiple reaction monitoring (MRM) transitions were measured in the positive ion mode. Validation of the method showed standard curves to be linear in the range of 10-4000 ng mL-1 for CIP and 30-12000 ng mL-1 for MET with mean correlation coefficient exceeding 0.999. In human plasma, CIP and MET were stable for at least 36 days at –70 ± 5 °C, 6 hours at ambient temperature and after three freeze thaw cycles. After extraction from plasma, the samples were stable in auto sampler at 22 °C for 6 hours. The method was simple, specific, sensitive, precise, accurate and suitable for bioequivalence and pharmacokinetic studies.

ASMAA ABDELKERIM MANDOUR KHADR

Ramzia I. El-Bagary, Asmaa A. El-Zaher, Ehab F. Elkady, Maha M. El-Hakim

01/02/2015

A stability indicating HPLC method for the simultaneous determination of ciprofloxacin HCl (CIP) and metronidazole (MET) in presence of ciprofloxacin acid degradation product (DEG) is presented (Method I, MI). The present study is concerned with the development and validation of an environmentally friendly method with relatively low organic composition of the mobile phase. The chromatographic separation of the two drugs was performed using Kromasil 100-5C18 (150 mm x 4.6 mm) with UV detection at 280 nm and flow rate of 1 mLmin-1. The mobile phase consisted of 0.5% aqueous phosphoric acid and acetonitrile (90:10 v/v) MI. In addition, another HPLC method (MII) for the separation of the same binary mixture using 0.5% aqueous phosphoric acid and acetonitrile (80:20 v/v) was presented. Retention times were 2.40, 3.10 & 22.94 min for MET, DEG and CIP, respectively, MI. On the other hand, the retention times were 2.03 and 4.00 min for MET and CIP, respectively MII. However, the relatively low organic composition of the mobile phase of 10% in MI was advantageous regarding green analytical procedure concept. Linearity, accuracy and precision were acceptable over the concentration range of 1-65 μgmL-1 for both drugs MI, and 1-80 μgmL-1 MII. The method is new, simple , sensitive and suitable for the routine quality control and dosage form assay of both drugs in the presence of the acid degradation product of ciprofloxacin. The method showed sufficient accuracy with a mobile phase of only 10% organic composition showing advantage and trying to approach green chromatographic conditions.